{"product_id":"9781493963300","title":"Artificial Riboswitches: Methods and Protocols","description":"Artificial riboswitches and other ligand—responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules.\u003ci\u003e Artificial Riboswitches: Methods and Prools\u003c\/i\u003e focuses on the state—of—the—art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, prools for evaluating the activities of the resultant riboswitches, as well as prools for construction of ligand—dependent, trans—acting gene regulators. Written in the successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step—by—step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.   \u003cp\u003e Authoritative and easily accessible, \u003ci\u003eArtificial Riboswitches: Methods and Prools\u003c\/i\u003e seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.  \u003c\/p\u003e","brand":"Springer-Verlag New York, LLC","offers":[{"title":"Default Title","offer_id":46351416819953,"sku":"9781493963300","price":139.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0674\/5433\/7265\/files\/9781493963300_p0.jpg?v=1765048756","url":"https:\/\/shop.barnesandnoble.com\/products\/9781493963300","provider":"Barnes \u0026 Noble","version":"1.0","type":"link"}