{"product_id":"9786208515492","title":"Molecular Biology Techniques: Polymerase Chain Reaction (PCR)","description":"The polymerase chain reaction (PCR) has as its main application the possibility of amplifying a segment of DNA, producing millions of copies of the selected segment. Gene cloning aims to introduce a selected gene into bacteria that are identical to each other, and this technique requires the presence of a cloning vector capable of amplifying a fragment of DNA cloned into them. The E gene of the dengue virus (DENV) is the most widely used for molecular studies due to the importance of the E protein. The aim of this study is to establish techniques for amplifying the E gene of DENV-2 and DENV-4. The DNA molecule was obtained from FIOCRUZ in Recife; the primers were selected in such a way as to encompass the E gene. PCR amplification was satisfactorily established for the E gene. Once determined, these techniques are essential in the stages of obtaining recombinant DNA in the process of cloning and expressing the dengue E protein.","brand":"Our Knowledge Publishing","offers":[{"title":"Default Title","offer_id":46312700084465,"sku":"9786208515492","price":37.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0674\/5433\/7265\/files\/9786208515492_p0.jpg?v=1765124800","url":"https:\/\/shop.barnesandnoble.com\/products\/9786208515492","provider":"Barnes \u0026 Noble","version":"1.0","type":"link"}